Level of 8-Hydroxy-2'Deoxyguanosine (8-OHdG) in children with acute lymphoblastic leukemia post-chemotherapy induction phase and post-chemotherapy maintenance phase

Anak Agung Ngurah Ketut  Putra Widnyana; Ni Kadek Wiwik Agustini; GAP Nilawati; I Made Gede Dwi Lingga; Wayan Dharma  Artana; I Made  Arimbawa; Ni Nyoman Metriani  Nesa

doi:10.15562/ism.v14i2.1702

Level of 8-Hydroxy-2'Deoxyguanosine (8-OHdG) in children with acute lymphoblastic leukemia post-chemotherapy induction phase and post-chemotherapy maintenance phase

Anak Agung Ngurah Ketut Putra Widnyana ,

Ni Kadek Wiwik Agustini ,

GAP Nilawati ,

I Made Gede Dwi Lingga ,

Wayan Dharma Artana ,

I Made Arimbawa ,

Ni Nyoman Metriani Nesa ,

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DOI: https://doi.org/10.15562/ism.v14i2.1702 |
Published: 2023-05-10

Abstract

Introduction: Chemotherapy is an important curative therapy modality in ALL patients. 8-Hydroxy-2'-Deoxyguanosine (8-OHdG) has been studied as a biomarker of oxidative stress as a trigger for DNA damage, which is statistically significantly increased in ALL patients after chemotherapy compared to healthy children. This study aims to prove that 8-OHdG levels in children with ALL post-chemotherapy induction phases were higher than in the post-chemotherapy maintenance phase.

Methods: This study used a cross-sectional design. The subjects were children aged 1-18 years, with ALL who received treatment at Prof Dr. I.G.N.G Ngoerah Hospital from September 2019-January 2021. The Mann-Whitney test assessed the 8-OHdG levels in children with ALL post-chemotherapy induction and post-chemotherapy maintenance phase. Logistic regression multivariate analysis was used to evaluate the factors that influence 8-OHdG levels in pediatric patients with ALL. The value of p<0,05 determined the level of significance.

Results: A total of 27 subjects who underwent the post-chemotherapy induction phase and 19 subjects who underwent the post-chemotherapy maintenance phase were analyzed in this study. In the post-chemotherapy induction group, the median 8-OHdG level was 0.163 (0.104-1.864), and the median 8-OHdG level in the post-chemotherapy maintenance phase group was 0.158 (0.094-0.512). There was no significant difference in 8-OHdG levels between the two groups. Logistic regression multivariate analysis found that the post-chemotherapy induction phase was a prognostic factor at risk of increasing 8-OHdG levels (RO 16.55; 95% CI 1.118-245.277).

Conclusion: This study concluded that 8-OHdG levels were higher in children post-chemotherapy induction phase than in children post-chemotherapy maintenance phase.

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