Gene similarity examination Pseudomonas aeruginosa in Intensive Care Unit (ICU) at Prof. Dr. dr. I.G.N.G Ngoerah hospital using the ERIC-PCR method: case reports

I Gede Wahyu Toya Pratama; Agus Eka Darwinata; Ni Made Adi Tarini

doi:10.15562/ism.v14i2.1656

Gene similarity examination Pseudomonas aeruginosa in Intensive Care Unit (ICU) at Prof. Dr. dr. I.G.N.G Ngoerah hospital using the ERIC-PCR method: case reports

I Gede Wahyu Toya Pratama ,

Agus Eka Darwinata ,

Ni Made Adi Tarini ,

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DOI: https://doi.org/10.15562/ism.v14i2.1656 |
Published: 2023-07-12

Abstract

Background: Pseudomonas aeruginosa is an opportunistic pathogen that has the potential to cause severe degrees of healthcare-associated infections, especially in immunocompromised patients who have AIDS, cancer, burns, and respiratory tract infections. Antibiotic resistance genes by genetic elements are increased so that it becomes a worldwide concern and also causes the evolution of resistant strains (MDR) ERIC-PCR technique is a PCR-based method in which the location and number of different ERIC sequences in bacteria are used as genetic markers of bacterial diversity.

Case Presentation: We presented three patients with Pseudomonas aeruginosa infection treated in the ICU (Intensive Care Unit) room of Prof. dr. I. G. N. G Ngoerah Hospital. While being treated in the ICU, the three patients' beds were located side by side in one room. Three patients, in this case, reports were treated in the same room where the beds were side by side and experienced a worsening of their condition. After examination, it was found that the bacteria that caused the infection was Pseudomonas aeruginosa. However, after conducting molecular analysis using the ERIC-PCR method, there was no gene similarity between the three isolates examined. This might have happened because the patient had been treated in a different room before being admitted to the ICU.

Conclusion: We presented a discrepancy between Pseudomonas aeruginosa infection in three patients treated in the same ICU room. However, the ERIC-PCR results found no gene similarity between the three isolates examined.

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